Dopaminergic lineage committed Neural Stem Cells (DA-NSCs) transitioned for differentiation into dopaminergic neurons. When thawed into Dopaminergic Differentiation Media and cultured in low oxygen conditions for 21-28 day these DA-NSCs will produce a robust population of functional neurons with 50-70% of the neurons being TH+ dopaminergic neurons as well as astrocytes and oligodendrocytes. Will last in culture for greater than 60 days. For best results should be cultured in low oxygen conditions.